Plymouth Proteomics Research Group

Centre of expertise in proteomics for biomedical applications
membrane protease, syn/degradation
The Plymouth Proteomics Research Group develops and applies advanced proteomic technologies to study human diseases addressing various research questions ranging from biomarker diagnostics to disease mechanisms.
Our key research areas are aligned with following core themes
  • Infection, immunity and inflammation
  • Clinical neuroscience
  • Cancer
We are involved in various internal and external projects with our expertise. If you are interested in proteomics service or a research collaboration, please contact Dr Vikram Sharma
 

Role of epigenetic modifications in cancer

Research work led by Dr Vikram Sharma focuses on post-translational modifications of histone/non-histone proteins and screening of novel epigenetic inhibitors in various cancer models.
Nucleosome, molecular model. The nucleosome consists of a DNA double helix wrapped around a core of histone proteins. Histone protein: smooth molecular surface model; DNA: cartoon model.
Nucleosome, molecular model
 

Proteomics facilities

  • LTQ Orbitrap Velos Pro (Thermo Fisher Scientific)
Ultra high resolution instrument used for peptide/protein identification purposes.
  • UHPLC- Dionex Ultimate 3000
A high performance nano-LC system coupled to LTQ Orbitrap Pro.
  • GE Healthcare Ettan TM IPGPhor3 for Isoelectric Focusing
A high performance platform used for protein fractionation.
Software available includes:
  • CLC Genomics Workbench
  • CLC Microbial Genome Finishing Module
  • Ingenuity Pathway Analysis
  • Proteome Discoverer
  • MaxQuant
Biomedical science laboratory 
 

Key publications

Malaria proteomics

Tremp AZ, Saeed S, Sharma V, Lasonder E & Dessens JT 2020 'Plasmodium berghei LAPs form an extended protein complex that facilitates crystalloid targeting and biogenesis' Journal of Proteomics 227, 103925-103925. Read this article: DOI
Green JL, Wu Y, Encheva V, Lasonder E, Prommaban A, Kunzelmann S, Christodoulou E, Grainger M, Truongvan N & Bothe S 2020 'Ubiquitin activation is essential for schizont maturation in Plasmodium falciparum blood-stage development' PLOS Pathogens 16, (6) e1008640-e1008640. Read this article: DOI PEARL
Bouyer G, Reininger L, Ramdani G, D Phillips L, Sharma V, Egee S, Langsley G & Lasonder E 2016 'Plasmodium falciparum infection induces dynamic changes in the erythrocyte phospho-proteome' Blood cells, molecules & diseases 58, 35-44. Read this article: https://doi.org/10.1016/j.bcmd.2016.02.001, PEARL
Saeed S, Tremp AZ, Sharma V, Lasonder E & Dessens JT 2020 'NAD (P) transhydrogenase has vital non‐mitochondrial functions in malaria parasite transmission' EMBO reports 21, (3). Read this article: DOI PEARLSuárez-Cortes P, Sharma V, Bertuccini L, Costa G, Bannerman NL, Sannella AR, Williamson K, Klemba M, Levashina EA & Lasonder E 2016 'Comparative proteomics and functional analysis reveal a role of P. falciparum osmiophilic bodies in malaria parasite transmission'
Molecular & cellular proteomics : MCP, doi: Read this article: 10.1074/mcp.M116.060681, PEARL

Membrane proteome dynamics

Valionyte E, Yang Y, Griffiths SA, Bone AT, Barrow ER, Sharma V, Lu B & Luo S (2021) 'The caspase-6–p62 axis modulates p62 droplets based autophagy in a dominant-negative manner' Cell Death & Differentiation , DOI Open access
C. Ramallo Guevara, O. Philipp, A. Hamann, A. Werner, H.D. Osiewacz, S. Rexroth, M. Rogner, A. Poetsch (2016) 'Global Protein Oxidation Profiling Suggests Efficient Mitochondrial Proteome Homeostasis During Aging' , Mol Cell Proteomics 15, (5) 1692-1709. Read this article DOI: 10.1074/mcp.M115.055616.
C. Trotschel, A. Poetsch (2015) 'Current approaches and challenges in targeted absolute quantification of membrane proteins' Proteomics 15, (5-6) 915-929. Read this article doi: 10.1002/pmic.201400427.
M. Cerletti, R.A. Paggi, C.R. Guevara, A. Poetsch, R.E. De Castro (2015) 'Global role of the membrane protease LonB in Archaea: Potential protease targets revealed by quantitative proteome analysis of a lonB mutant in Haloferax volcanii', J Proteomics 121, 1-14. Read this article doi: 10.1016/j.jprot.2015.03.016.
C. Trotschel, S.P. Albaum, D. Wolff, S. Schroder, A. Goesmann, T.W. Nattkemper, A. Poetsch (2012) 'Protein turnover quantification in a multilabeling approach: from data calculation to evaluation' Mol Cell Proteomics 11, (8) 512-526. Read this article doi: 10.1074/mcp.M111.014134.
A. Poetsch, D. Wolters (2008) 'Bacterial membrane proteomics' Proteomics 8, 4100-4122. Read this article doi: 10.1002/pmic.200800273.

Disease biology, cancer research and epigenetics

Dunn J, Ferluga S, Sharma V, Futschik M, Hilton DA, Adams CL, Lasonder E & Hanemann CO 2018 'Proteomic analysis discovers the differential expression of novel proteins and phosphoproteins in meningioma including NEK9, HK2 and SET and deregulation of RNA metabolism' EBioMedicine. Author Site , Read this article: DOI PEARL
Eymael J, Sharma S, Loeven MA, Wetzels JF, Mooren F, Florquin S, Deegens JK, Willemsen BK, Sharma V & van Kuppevelt TH 2017 'CD44 is required for the pathogenesis of experimental crescentic glomerulonephritis and collapsing focal segmental glomerulosclerosis' Kidney International 93, (3) 626-642 , Read this article: DOI PEARL
Sareen N, Sequiera GL, Chaudhary R, Abu-El-Rub E, Chowdhury SR, Sharma V, Surendran A, Moudgil M, Fernyhough P & Ravandi A 2018 'Early passaging of mesenchymal stem cells does not instigate significant modifications in their immunological behavior' Stem Cells Research and Therapy. Read this article: DOI PEARL
Zhou L, Lyons-Rimmer J, Ammoun S, Müller J, Lasonder E, Sharma V, Ercolano E, Hilton D, Taiwo I & Barczyk M 2015 'The scaffold protein KSR1, a novel therapeutic target for the treatment of Merlin-deficient tumors' Oncogene 35, (26) 3443-3453. Read this article: DOI PEARL
Middeljans E, Wan X, Jansen PW, Sharma V, Stunnenberg HG & Logie C 2012 'SS18 together with animal-specific factors defines human BAF-type SWI/SNF complexes' Plos One. Read this article: https://doi.org/10.1371/journal.pone.0033834
Shahhoseini M, Favaedi R, Baharvand H, Sharma V & Stunnenberg HG 2010 'Evidence for a dynamic role of the linker histone variant H1x during retinoic acid-induced differentiation of NT2 cells' FEBS Letters 4661-4664. Read this article: ttps://doi.org/10.1016/j.febslet.2010.10.041
 

Our team

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